Ali Maged Ali Awaad
Al-Azhar University, Egypt
Posters & Accepted Abstracts: J Adv Dairy Res
Bifidobacterium represent one of the major genera of the intestinal tract of human and animals and are used, in probiotics, in dairy and non-dairy foods to restore the intestinal microflora which confer a health benefit. The identification of Bifidobacterium by phenotypic features is commonly unreliable and also time, money and effort consuming. In order to improve identification methods decreasing time, money and effort consuming and precise based on molecular level to identify of probiotic bacteria in complex microbial communities, the application of 16S-23S rRNA oligonucleotide probes is the best and most reliable approach in rapid and precise identification of species. The 16S rRNA gene was used for the systematic identification of bifidobacteria. In this study, we used seven primer pairs on complex communities to facilitate the examination of complex micro ecosystems. The 2 primers Bif162-Bif662 and Bflac2-Bflac5 showed positive results with Bifidobacterium spp. and B.animalis subsp lactis, these strains were isolated from breast milk of human and feces of breast fed infants. The 2 DNA fragments were sequenced and submitted in GenBank "NCBI" under accessions number "KT758845"and "KT758846". The long term goal of this research is to obtain patent protection for Bifidobacterium sp which isolated from Egyptian resources and use it in food and dairy products.
Email: ali.maged2010@gmail.com
