Jamuna R. Subramaniam
Accepted Abstracts: J Cell Sci Ther
Human umbilical cord is a highly abundant, non controversial source with tremendous potential for Mesenchyma stem cells (MSCs). We derived matrix stromal cells (HUMS) from the human umbilical cord. Th e HUMS cells secrete several neurotrophic factors. Th e HUMS cells and their secreted factors are shown to provide some amount of neuroprotection in the neurodegenerative disease models of mice. But the exact mechanism of protection is not well understood. Here, we report that the HUMS cells -secrete six neurotrophic factors, namely, NT-3, NGF, BDNF, VEGF, IGF-1 and GDNF(NFs). Th ese NFs present in the conditioned medium of the HUMS cells induce diff erentiation, neurite extension and neural networking of a motor neuron cell line, NSC34. Th ese motor neurons express the tyrosine kinase receptors for the above trophic factors (except for BDNF), which are crucial for neurite extension. Th e tyrosine kinase inhibitor, K252a, drastically reduces CM induced neurite extension. Further, all the 5 TFs need to be neutralized simultaneously with their antibodies to abrogate neurite extension, proving the fl exibility and prudent backup mechanism of the system. Intriguingly, none of the phenomenon ? diff erentiation, neurite extension or neural networking required cAMP second messenger system coupling as evidenced by cAMP pathway activator or inhibitor treatment of the NSC34 cells with or without CM
Jamuna R. Subtamaniam has completed her Ph.D. from Georgetown University, Washington DC and PDF from Johns Hopkins University, Baltimore. Currently, she is Senior Research Scientist in Indian Institute of Technology Kanpur, India. She has around 17 publications in reputed journals.
