Soon Ho Hong
Posters-Accepted Abstracts: J Fundam Renewable Energy Appl
Escherichia coli were engineered for the direct production of gamma-aminobutyric acid from glucose by introduction of
synthetic protein scaffold. In this study, three enzymes consisting GABA pathway (isocitrate dehydrogenase, glutamate
synthase and glutamate decarboxylase) were connected via synthetic protein scaffold. By introduction of scaffold, 0.92 g/L
of GABA was produced from 10 g/L of glucose while no GABA was produced in wild type E. coli. The optimum pH and
temperature for GABA production were 4.5 and 30oC, respectively. When competing metabolic network was inactivated by
knockout mutation, maximum GABA concentration of 1.3 g/L was obtained from 10 g/L glucose. The recombinant E.coli
strain which produces GABA directly from glucose was successfully constructed by introduction of protein scaffold.
