Md Meskatul Alam, Kazuhiko Shimasaki, Sultana Umma Habiba and Jobyda Nasrin
Ehime University, Japan
Dhaka International University, Bangladesh
Posters & Accepted Abstracts: J Neurosci Neuropharm
Light is one of the most important abiotic factors, acting on plants as the sole source of energy for plants growth and development. In this study, protocorm-like bodies (PLBs) of Phalaenopsis were cultured in modified MS medium to see the potential effect of BAP on organogenesis of PLBs under blue, green and red light emitting diodes (LEDs) and responses were compared with explants grown under fluorescent light at 16 h photoperiod. The maximum average number of PLBs (14.27) and fresh weight (0.22 g) per explant was found in the medium containing 0.01 mg/L BAP under red LEDs. Whereas, shoot formation was maximum (4.5/explant) in red LED at 1 mg/L BAP, in case of blue LED no satisfactory results were found on Phalaenopsis PLBs multiplication compared with white light. These results suggest that this newly developed light source could be used as an energy efficient light source for the organogenesis of Phalaenopsis PLBs in vitro and red LED would be more effective on PLBs and shoot formation.
