Hassanzadeh S, Jameie SB, Mehdizadeh M, Soleimani M, Farhadi M, Soleimani Maryam, Mollajani R, Jameie Mana and Jameie Melika
Iran University of Medical Sciences, Iran
University of social Welfare and Rehabilitation Sciences, Iran
Shahid Beheshti University of medical Sciences, Iran
Posters & Accepted Abstracts: J Neurol Neurophysiol
Spinal cord injury is suffering from the medical condition that might happen to anyone. Therapy mainly focuses on rehabilitation and pharmacological treatment. Supra-spinal changes in the cerebellum that receives afferents from the spinal cord might be the reason for unsuccessful therapy. The expression of FNDC5 was reported in cerebellar Purkinje cells. In the present study, we considered the expression of FNDC5 in Purkinje cells following SCI with and without MP administration in adult rat with SCI. Thirty-five adult male rats were used. The animals randomly allocated in five groups, including SCI, spinal cord injury with methylprednisolone treatment, operation sham, control and operation sham with MP. SCI was done by using the special clip to compress the spinal cord at the supposed level. Then the animals went understudy for FNDC5 expression, apoptosis by using immunohistochemistry, western blotting, TUNEL and Nissl staining. Our results showed the significant decrease in the number of Purkinje cells following SCI. Therapy with MP inhibits the apoptosis in irFNDC5 Purkinje cells and restore them. Expression of FNDC5 significantly increased in SCI and decreased following MP therapy. We also showed other cerebellar cells with FNDC5 immunoreactivity in the two other cerebellar layers that are firstly reported. Since the irisin is known as the plasma product of FNDC5 we think that it might be a plasma marker following therapeutic efforts of SCI, however, it needs further research. In addition, it is possible that changes in FNDC5 expression in Purkinje cells might be related to neurogenesis in cerebellum with unknown mechanisms.
E-mail: behjame@gmail.com
