Ulger C and Orenlili yaylagul E
Adnan Menderes University Faculty of Arts & Science Department of Biology Molecular Biology, Turkey
Posters & Accepted Abstracts: J Cell Signal
Osteosarcoma is the most common primary bone malignancy which occurs frequently in children and adolescents. The flavonoid baicalein, shows a wide range of biological and pharmaceutical effects, with its potent antitumor activity raising great interest in recent years. MicroRNAs are emerging as a class of small regulatory RNAs whose alterations are implicated in the initiation and progression of human cancers. MicroRNA-25 (miR-25) has recently been found to be involved in many critical processes in human cancers. In this study, the effect of baicalein and miR-25 on Wnt/���²-catenin signaling pathway in osteosarcoma cell line Saos-2 was investigated. Cell viability and apoptosis was assessed, mRNA and protein expressions of ���²-catenin, GSK-3���², Axin2 and LRP-5, which are associated with the Wnt/���²-catenin signaling pathway, were determined. In Saos-2 cells, baicalein IC50 and AP50 values were found to be 35 ���¼M ve 50 ���¼M, respectively. It is indicated that baicalein can inhibit the proliferation and induce apoptosis and also increase miR-25 expression of Saos-2 cells in a dose-dependent manner. Baicalein treatment and miR-25 mimic transfection decreased the expression of ���²-catenin, Axin2 and LRP-5, while increasing the expression of GSK-3���². Anti-miR-25 treatment decreased the expression of GSK-3���² and LRP-5, while ���²-catenin and Axin2 expression increased. These findings demonstrate that baicalein may target genes related to the Wnt/���²-catenin pathway by regulating miR-25 expression and may be a potential Wnt/���²-catenin pathway inhibitor for osteosarcoma therapy.
E-mail: culger@adu.edu.tr
