Weam Ahmed Maher Rashwan
Cairo University, Egypt
Posters-Accepted Abstracts: J Clin Cell Immunol
Background: Tumor Necrosis Factor alpha (TNF-alpha) and lymphotoxin alpha are pivotal cytokines in the pathogenesis of systemic lupus erythmatosis. Objectives: To investigate the possible association of the polymorphism of the TNF promoter gene at position -308 and that of the LTA gene at position 252 with susceptibility to SLE and with phenotypic disease features in Egyptians patients. Subjects and Methods: A case control study involving 100 SLE patients and 100 unrelated healthy controls. Polymerase chain reaction and restriction fragment length (PCR-RFLP) methods were applied to detect polymorphism in TNF Promoter (-308 G>A) and LTA 252 A>G. Results: We found TNF- 308 genotype AA was significantly increased by 26% in SLE patients compared to 10% in the control group and genotype LTA 252 GG showed a significant increase by 22% in SLE patients compared to 6% in the control group. Mutant allele A of TNF and mutant allele G of LTA were significantly associated with SLE (p<0.001, OR=2.29, 2.31 and CI=1.49- 3.52, 1.48-3.6 respectively). Genotype (AA+GA) of TNF was significantly associated with clinical manifestations as malar rash, arthritis, oral ulcers,serositis and systemic lupus erythematosus disease activity index(SLEA1). Genotype (GG+GA) of LTA was significantly associated with arthritis. Conclusion: These results suggest that TNF and LTA genetic polymorphisms contribute to SLE susceptibility in the Egyptian population and are associated with disease characteristics.
Email: weamrashwan@yahoo.com
